SHIKARI® T-CAP Nab Assay™-Bevacizumab ELISA Kit
BEV-TCAP-NAb-AA
Target Capture Neutralizing Antibodies Immunoassay-Bevacizumab
Enzyme immunoassay to detect neutralizing antibodies to bevacizumab in serum and plasma samples
This kit has been developed for detecting ‘Free’ neutralizing antibodies to bevacizumab. The kit is composed of 4 different controls,
- Positive control: It contains netralizing antibody
- Negative control
- Cut-Off control: Contains certain amounted neutralizing antibody. Corresponse to lower limit of detection (LLOD)
- Non-Neutralizing Antibody Negative Control: Contains non-neutralizing antibodies binding to the drug
With these controls, the kit provides reliable results.
The Cut Off serum contains the LLOD amount and provides accurate true positive or negative results.
A non-neutralising antibody that binds to the drug without affecting the test results can be considered as a negative control.
The kit was also checked with various negative serum samples, including RF(+), CRP(+), ANA(+) sera. All these negative sera did not interfere with the test system. Please refer to the validation report.
We strictly recommend taking sample just before the next dose administration from the patient.
All SHIKARI® ELISA kits are produced under ISO 13485 quality system and have CE IVD mark.
For technical inquiry, please contact techsupport@matriksbiotek.com
| Required Volume (µl) | 10 |
| Total Time (min) | 140 |
| Sample | Serum, plasma |
| Sample Number | 96 |
| Detection Limit (ng/mL) | 20 |
| Spike Recovery (%) | - |
| Shelf Life (month) | 6 |
| Assay type | Qualitative |
| Species Reactivity | Human |
| Storage conditions | Store at +4°C. Please refer to protocols. |
| Shipping conditions | At room temperature |
| # | File | Action |
|---|---|---|
| Instructions for Use | Download | |
| SDS | Download | |
| Validation Report | Download |
Publications with this drug
| # | File | Action |
|---|---|---|
| Formica, María Lina, et al. "Novel hybrid lipid nanocapsules loaded with a therapeutic monoclonal antibody–Bevacizumab–and Triamcinolone acetonide for combined therapy in neovascular ocular pathologies." Materials Science and Engineering: C 119 (2020): 111398. | Visit Link | |
| Luis de Redín, Inés, et al. "In vivo efficacy of bevacizumab-loaded albumin nanoparticles in the treatment of colorectal cancer." Drug Delivery and Translational Research 10 (2020): 635-645. | Visit Link | |
| Llabot, Juan M., et al. "In vitro characterization of new stabilizing albumin nanoparticles as a potential topical drug delivery system in the treatment of corneal neovascularization (CNV)." Journal of Drug Delivery Science and Technology 52 (2019) | Visit Link | |
| Gregoritza, Manuel, et al. "Fabrication of antibody-loaded microgels using microfluidics and thiol-ene photoclick chemistry." European Journal of Pharmaceutics and Biopharmaceutics 127 (2018): 194-203. | Visit Link | |
| de Redín, Inés Luis, et al. "Human serum albumin nanoparticles for ocular delivery of bevacizumab." International Journal of Pharmaceutics 541.1-2 (2018): 214-223. | Visit Link | |
| Gojo, Johannes, et al. "Pharmacokinetics of bevacizumab in three patients under the age of 3 years with CNS malignancies." Drugs in R&D 17 (2017): 469-474. | Visit Link | |
| Gregoritza, Manuel, et al. "Controlled antibody release from degradable Thermoresponsive hydrogels cross-linked by Diels–Alder chemistry." Biomacromolecules 18.8 (2017): 2410-2418. | Visit Link | |
| Chen, Lei, et al. "Efficient production of a bioactive bevacizumab monoclonal antibody using the 2A self-cleavage peptide in transgenic rice callus." Frontiers in plant science 7 (2016): 1156. | Visit Link | |
| Van Bergen, Tine, et al. "Complementary effects of bevacizumab and MMC in the improvement of surgical outcome after glaucoma filtration surgery." Acta Ophthalmologica 93.7 (2015): 667-678. | Visit Link | |
| Akbulut, Hakan, et al. "The trough levels of bevacizumab significantly affect the outcome of the treatment in patients with metastatic colorectal cancer: A Turkish Oncology Group study." (2018): e15553-e15553. | Visit Link | |
| Akbulut, H., et al. "The role of immune system on the efficacy of bevacizumab in patients with metastatic colorectal cancer (mCRC)." Annals of Oncology 27 (2016): vi537. | Visit Link | |
| Peña Cabia, Silvia. "Evaluación de la relación exposición-respuesta de bevacizumab y de cetuximab en cáncer colorrectal metastásico y de cetuximab en cáncer de cabeza y cuello." (2022). | Visit Link | |
| Legeron, Rachel. La spectrométrie de masse appliquée à la quantification absolue des anticorps monoclonaux thérapeutiques en milieu plasmatique pour la réalisation d'études pharmacocinétiques-pharmacodynamiques. Diss. Université de Bordeaux, 2015. | Visit Link | |
| Uğurlu, Nagihan. "Nanoteknoloji Bazlı İlaç Taşıma Sistemlerinin Göze İlaç Uygulamalarında, Transkleral Geçiş Üzerine Etkisinin Değerlendirilmesi." (2013). | Visit Link | |
| Ramos Membrive, Rocío. Radiomarcaje y biodistribución mediante SPECT/CT de nanopartículas de seroalbúmina humana con bevacizumab. Diss. Universidad de Navarra, 2020. | Visit Link | |
| Paul, B. Yu, and Marcelo Dicarli. "Imaging probe for angiogenic activity in pulmonary arterial hypertension." U.S. Patent Application No. 15/770,994. | Visit Link | |
| The plant source recombinant humanized shellfish of a kind of optimization cuts down preparation method and the medical applications of monoclonal antibody, 于为常陈磊 | Visit Link | |
| Pangua, Cristina, et al. "Mucus-penetrating and permeation enhancer albumin-based nanoparticles for oral delivery of macromolecules: Application to bevacizumab." Drug Delivery and Translational Research 14.5 (2024): 1189-1205. | Visit Link | |
| Akay Hacan, Büşra. "Rutin tedavi olarak bevacizumab kullanılan metastatik kolorektal kenserli kolorektal kanserli hastalarda tedavi etkinliğinin belirlenmesinde biyobelirteçlerin rolü." | Visit Link | |
| Pangua, Cristina, et al. "Enhancing bevacizumab efficacy in a colorectal tumor mice model using dextran-coated albumin nanoparticles." Drug Delivery and Translational Research (2024): 1-18. | Download |
